Annals of African Medicine
Home About AAM Editorial board Ahead of print Current Issue Archives Instructions Subscribe Contact us Search Login 
ORIGINAL ARTICLE
Year : 2008  |  Volume : 7  |  Issue : 4  |  Page : 163-167

Electropherotypes and subgroups of group A rotaviruses circulating among diarrhoeic children in Kano, Nigeria


1 Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria
2 Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
3 MRC/MEDUNSA Diarrheal Pathogens Research Unit, Medical University of South Africa, Pretoria, South Africa

Correspondence Address:
A A Dzikwi
Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria
Nigeria
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1596-3519.55659

Rights and Permissions

Background: It is estimated that about 600 000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. The virus is a double stranded RNA (dsRNA) virus with 11 segments. Group A rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. The VP6 subgroups, I and II exist. This work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in Kano, and to determine the circulating subgroups and electropherotypes and of the rotavirus isolates. Methods: Two hundred and eighteen stool specimens from children 0-60 months (198 diarrheic and 20 non-diarrheic) were collected from different hospitals and health care centers in Kano and subjected to group A rotavirus enzyme linked immunosorbent assay (ELISA) to determine presence of group A rotavirus, subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present. Results: The long electropherotypes (47.05%) of four variations dominated over the short electropherotype (17.64%). About 11.76% of the isolates were of mixed infection. Dominance of subgroup II (45%) over subgroup I (25%), and the presence of both subgroups I and II (10%) and neither subgroup I nor II (15%) was observed in this study. Conclusion: Information on the genomic diversity of the RNA electropherotypes in this region, Kano, is reported in this study.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed4147    
    Printed163    
    Emailed1    
    PDF Downloaded78    
    Comments [Add]    
    Cited by others 1    

Recommend this journal